Number of amino acids: 262
Molecular weight: 28316.5
Theoretical pI: 5.71
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 1269
Hydrogen H 2000
Nitrogen N 360
Oxygen O 358
Sulfur S 9
Formula: C1269H2000N360O358S9Total number of atoms: 3996
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 42190
Abs 0.1% (=1 g/l) 1.490, assuming ALL Cys residues appear as half cystines
Ext. coefficient 41940
Abs 0.1% (=1 g/l) 1.481, assuming NO Cys residues appear as half cystines
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.03
This classifies the protein as unstable.
Aliphatic index: 104.27
Grand average of hydropathicity (GRAVY): 0.023
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Please note the modified algorithm for extinction coefficient.