Number of amino acids: 441
Molecular weight: 45866.1
Theoretical pI: 10.63
Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 2106
Hydrogen H 3360
Nitrogen N 578
Oxygen O 544
Sulfur S 12
Formula: C2106H3360N578O544S12Total number of atoms: 6600
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 57075
Abs 0.1% (=1 g/l) 1.244, assuming ALL Cys residues appear as half cystines
Ext. coefficient 56950
Abs 0.1% (=1 g/l) 1.242, assuming NO Cys residues appear as half cystines
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 26.02
This classifies the protein as stable.
Aliphatic index: 125.22
Grand average of hydropathicity (GRAVY): 0.840
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Please note the modified algorithm for extinction coefficient.