Number of amino acids: 281
Molecular weight: 30308.4
Theoretical pI: 11.66
Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1315
Hydrogen H 2150
Nitrogen N 444
Oxygen O 377
Sulfur S 3
Formula: C1315H2150N444O377S3Total number of atoms: 4289
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 26930
Abs 0.1% (=1 g/l) 0.889, assuming ALL Cys residues appear as half cystines
Ext. coefficient 26930
Abs 0.1% (=1 g/l) 0.889, assuming NO Cys residues appear as half cystines
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.75
This classifies the protein as unstable.
Aliphatic index: 90.75
Grand average of hydropathicity (GRAVY): -0.288
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Please note the modified algorithm for extinction coefficient.