Number of amino acids: 469
Molecular weight: 50610.8
Theoretical pI: 9.78
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 2355
Hydrogen H 3741
Nitrogen N 605
Oxygen O 612
Sulfur S 9
Formula: C2355H3741N605O612S9Total number of atoms: 7322
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 55015
Abs 0.1% (=1 g/l) 1.087, assuming ALL Cys residues appear as half cystines
Ext. coefficient 54890
Abs 0.1% (=1 g/l) 1.085, assuming NO Cys residues appear as half cystines
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.99
This classifies the protein as stable.
Aliphatic index: 125.44
Grand average of hydropathicity (GRAVY): 0.677
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Please note the modified algorithm for extinction coefficient.