Number of amino acids: 232
Molecular weight: 24626.9
Theoretical pI: 6.02
Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 14
Atomic composition:
Carbon C 1093
Hydrogen H 1721
Nitrogen N 311
Oxygen O 326
Sulfur S 6
Formula: C1093H1721N311O326S6Total number of atoms: 3457
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 21220
Abs 0.1% (=1 g/l) 0.862, assuming ALL Cys residues appear as half cystines
Ext. coefficient 20970
Abs 0.1% (=1 g/l) 0.852, assuming NO Cys residues appear as half cystines
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.46
This classifies the protein as stable.
Aliphatic index: 96.68
Grand average of hydropathicity (GRAVY): 0.128
ExPASy Home page
Please note the modified algorithm for extinction coefficient.