PROSO

A sequence-based PROtein SOlubility evaluator.

PROSO tries to answer the following questions:

"Which of my cloned proteins have the best/worst chances tobe soluble upon heterologous expression?"



RESULTS

The input protein sequences are categorized to classes:
YES - soluble or NO - insoluble.
Additionally the probability of a class (from 0.5 to 1.0) is provided.

The probability threshold value is set by default to 0.5. By increasing it one can expect higher classification precision (selectivity) and lower recall (sensitivity) (more details in Method Description).
 
If the result cannot be calculated, a comment is written.


Prot. nameProt. seq.Result:
Class; Class probability
Rv2225 MSEQTIY...

yes;      0.618

Rv2226 MPVEAPR...

no;      0.754

Rv2227 MGQTRRL...

NO;      0.748

Rv2228c MKVVIEA...

no;      0.823

Rv2229c MKAGVAQ...

yes;      0.749




If you use this server, please cite:
Smialowski P, Martin-Galiano AJ, Mikolajka A, Girschick T, Holak TA and Frishman D. Protein solubility: sequence based prediction and experimental verification. Bioinformatics. 2006 Dec 6; [Epub ahead of print]


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Last modified Apr 04, 2006