PROSO

A sequence-based PROtein SOlubility evaluator.

PROSO tries to answer the following questions:

"Which of my cloned proteins have the best/worst chances tobe soluble upon heterologous expression?"



RESULTS

The input protein sequences are categorized to classes:
YES - soluble or NO - insoluble.
Additionally the probability of a class (from 0.5 to 1.0) is provided.

The probability threshold value is set by default to 0.5. By increasing it one can expect higher classification precision (selectivity) and lower recall (sensitivity) (more details in Method Description).
 
If the result cannot be calculated, a comment is written.


Prot. nameProt. seq.Result:
Class; Class probability
Rv0317c MEFLRHG...

no;      0.893

Rv0318c MSLAVTM...

no;      0.823

Rv0319 MSKVLVT...

NO;      0.697

Rv0320 MGRHELA...

yes;      0.587

Rv0321 MLLSDRD...

yes;      0.539




If you use this server, please cite:
Smialowski P, Martin-Galiano AJ, Mikolajka A, Girschick T, Holak TA and Frishman D. Protein solubility: sequence based prediction and experimental verification. Bioinformatics. 2006 Dec 6; [Epub ahead of print]


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Last modified Apr 04, 2006