The mutants have been constructed using the specialized transduction phage strategy.
More information here.
Cosmid information here
| Knockout Targets |
|---|
| Target / length | Gene product | Transduction Phage | Knockout Strain | Complementing Plasmid | Primers | PCR | Sequencing | Cosmid | Southern | Comments |
|---|
| Rv0007 / 2913 | Rv0007 | phAE10007 | mc210007 | | | | | | | |
| Rv0008c / 2436 | Rv0008c | phAE100008 | mc210008 | | | | | | | |
| Rv0036c / 774 | Rv0036c | phAE10036 | mc210036 | MT1AQ35 (2H2) | | | | | | |
| Rv0037c / 1326 | Rv0037c | phAE10037 | mc210037 | MT1AQ35 (2H2) | yes | | no | | | |
| Rv0038 / 609 | Rv0038 | phAE10038 | mc210038 | MT1AQ35 (2H2) | yes | | | | | |
| Rv0039c / 348 | Rv0039c | phAE10039 | mc210039 | MT1AQ35 (2H2) | | | no | | | |
| Rv0040c / 933 | mtc28 | phAE10040 | mc210040 | MT1AQ35 (2H2) | | | | | | |