Rv3488, (MTCY13E12.41), len: 107 aa. Hypotheticalprotein, similar to various bacterial proteins e.g.O28730|AF1542 CONSERVED HYPOTHETICAL PROTEIN fromArchaeoglobus fulgidus (101 aa), FASTA scores: opt: 321,E(): 6.4e-15, (50.55% identity in 87 aa overlap); O50207SQ1_IV (FRAGMENT) from Rhodococcus erythropolis (59 aa),FASTA scores: opt: 298, E(): 1.4e-13, (71.2% identity in59 aa overlap); Q9KFB0|BH0575 BH0575 PROTEIN from Bacillushalodurans (102 aa), FASTA scores: opt: 294, E(): 4.1e-13,(43.15% identity in 95 aa overlap); etc. Also similar toMycobacterium tuberculosis P71704|Rv0047c|MTCY21D4.10c(180 aa) (37.8% identity in 82 aa overlap).
Rv3490: INVOLVED IN OSMOREGULATORY TREHALOSEBIOSYNTHESIS. Mycobacteria can produce trehalose fromglucose 6-phosphate and UDP-glucose (the OtsA-OtsBpathway) from glycogen-like alpha(1-->4)-linked glucosepolymers (the TreY-TreZ pathway) and from maltose (theTreS pathway) [CATALYTIC ACTIVITY: UDP-GLUCOSE + D-GLUCOSE6-PHOSPHATE = UDP + ALPHA,ALPHA-TREHALOSE 6-PHOSPHATE].
Rv3490, (MTCY13E12.43), len: 500 aa. Probable otsA,alpha, alpha-trehalose-phosphate synthase (EC 22.214.171.124)(see citations below), equivalent to Q50167|OTSA|ML2254PROBABLE TREHALOSE-PHOSPHATE SYNTHASE from Mycobacteriumleprae (498 aa), FASTA scores: opt: 2706, E(): 1.6e-166,(80.3% identity in 497 aa overlap). Also similar to otherse.g. Q92410|TPS1_CANAL from Candida albicans (Yeast) (478aa), FASTA scores: opt: 895, E(): 4.9e-50, (37.15%identity in 479 aa overlap);Q00764|TPS1_YEASTTPS1|CIF1|BYP1|FDP1|GGS1|GLC6|YBR126c|YBR0 922 from Saccharomyces cerevisiae (Baker's yeast) (495aa), FASTA scores: opt: 847, E(): 6.2e-47, (36.1% identityin 490 aa overlap); BAB48232|MLL0691 from Rhizobium loti(Mesorhizobium loti) (520 aa), FASTA scores: opt: 884,E(): 2.7e-49, (36.2% identity in 478 aa overlap); etc.Equivalent to AAK47953 from Mycobacterium tuberculosisstrain CDC1551 (478 aa) but longer 22 aa.